ITCH
ITCH, also called atrophin-1 interacting protein 4 (AIP4), is a HECT-type E3 ubiquitin ligase. It contains an N-terminal Ca-dependent phospholipid-binding C2 domain, four WW domains, and a C-terminal HECT domain. Mice deficient in ITCH protein display many autoimmune-like disease characteristics such as lymphoproliferation in the spleen and lymph nodes. More than a dozen of substrates of ITCH have been identified including p63, p73 and Notch1. The ligase may be responsible for ubiquitinating proteins with K29-, K48-, and/or K63-linked ubiquitin chains.
Product Name:
ITCH
>Also Known As:
AIF4; AIP4; NAPP1; dJ468O1.1
>Catalog No.:
K1400
>Size:
50 µg
>Molecular Weight:
102.8 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~90% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_001257137
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified ITCH |
>Shipping Method:
Dry ice shipping
>References:
1. Perry WL, et al. (1998) Nat Genet 18, 143 – 146.
2. Schwarz SE, et al. (1998) J Biol Chem 273, 12148 – 12154.
3. Melino G, et al. (2008) Cell Death Differ 15(7), 1103 – 1112. AdipoRon 1847500
