IPTG
IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in E. coli. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.
Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Sterile the solution using a 0.22 um syringe filter. Aliquot the stock and store under -20°C for up to one year.
Product Name:
IPTG
>Also Known As:
isopropyl – β – D – thiogalactopyranoside
>Catalog No.:
P1010-10
>Size:
10 g
>CAS:
367-93-1
>Formula:
C9H18O5S
>Molecular Weight:
238.3
>Form:
Powder
>Quality Assurance:
>99% by HPLC, dioxane-free
>Source:
Synthetic
>Storage:
Eligible for ambient temperature shipping. Store at 4°C upon receiving.
>PDF Data Sheet:
PDF Datasheet
>Image(s):
>Shipping Method:
Room temperature shipping
>References:
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125. BIBF 1120 18472341
