GST-Ubiquitin(1-75)
Ub75 lacks the amino acid glycine 76, which is required to form thioester bond with Ub activating enzyme E1. Therefore, Ub75 can not react with E1, E2 and E3 enzymes; and does not support ubiquitination. It can be used as a negative control in ubiquitination reactions; or to assay binding of Ub with Ub binding proteins or ubiquitination enzymes. This product can be used in pulldown assays to determine ubiquitin-binding proteins.
Product Name:
GST-Ubiquitin(1-75)
>Also Known As:
GST-Ub(1-75)
>Catalog No.:
E1210
>Size:
1 mg
>Molecular Weight:
8.5 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-Ubiquitin(1-75) |
>Shipping Method:
Dry ice shipping
>References:
1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.
2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.
5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228. AZD3965 18477041
