Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)
Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively.
When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Product Name:
Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)
>Also Known As:
Boc-LRR-AMC
>Catalog No.:
G3100
>Size:
5 mg
>Molecular Weight:
773.8 Da
>Species:
N/A
>Source:
Synthetic
>Stock:
Powder
>Concentration:
N/A
>Quality Assurance:
>98% by HPLC and NMR
>Storage:
Eligible for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.
>PDF Data Sheet:
Download PDF datasheet
>NCBI RefSeq:
N/A
>Image(s):
No
>Shipping Method:
Room temperature shipping
>References:
1. Chen P, et al. (1996) Cell 86(6), 961 – 972.
2. Arendt CS, et al. (1997) Proc Natl Acad Sci 94(14), 7156 – 7161.
3. Reidlinger J, et al. (1997) J Biol Chem 272(40), 24899 – 24905. Opicapone 18469671
