Anti-Ubiquitin (P4D1)
Anti-Ub Mouse Monoclonal Antibody
Product Name:
Anti-Ubiquitin (P4D1)
>Also Known As:
Anti-Ub
>Catalog No.:
Y3011
>Size:
100 µg
>Molecular Weight:
~55/25 kDa
>Species:
Recognizes ubiquitin in a wide range of species
>Source:
Immunogen: Full length bovine ubiquitin
>Stock:
lyophilized powder
>Concentration:
lyophilized powder
>Quality Assurance:
Guaranteed for detecting 5 ng endogenous ubiquitin or polyubiquitin chains in whole cell or tissue lysates at an antibody dilution of 1:1,000.
>Storage:
1) Store the lyophilized antibody desccated at -20°C. 2) After reconstitution, store the antibody at -20°C. Aliquot is not necessary. The quality of the antibody is guaranteed for one year after puchasing if stored correctly.
>PDF Data Sheet:
PDF Datasheet
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
Figure 1.Western blot applications
Anti-Ubiquitin (P4D1) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3-12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng. Lanes 6 & 7 represent 20 μg of Swiss 3T3 cell lysate from cells treated for 5h with 10 µM MG-132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.
Figure 2. IF of HeLa cells
HeLa cells were stained and visualized by fluorescence microscopy. Ubiquitin targeted cytoplasmic and nuclear proteins and free ubiquitin were stained using Anti-Ubiquitin (P4D1) at 1:500 dilution.
>Shipping Method:
Room temperature shipping
>References:
N/A PI-3065 18472174
