Anti-6xHis
Anti-His (HIS.H8) Mouse Monoclonal Antibody
Product Name:
Anti-6xHis
>Also Known As:
No
>Catalog No.:
Y1010
>Size:
25 µg
>Molecular Weight:
~55/25 kDa
>Species:
N/A
>Source:
Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH
>Stock:
10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
>Concentration:
1 µg/µl
>Quality Assurance:
WB (with ECL): 1-2 ng of His-tagged fusion proteins
>Storage:
Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)
Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2)
>Shipping Method:
Wet ice shipping
>References:
N/A Presatovir 18471059
