6xHis-USP16
USP16 is an active deubiquitinating enzyme (DUB) encoded on chromosome 21, trisomy of which causes Down’s syndrome (DS). USP16 is a phosphoprotein, phosphorylation of USP16 controls its cellular localization, but no effect on its DUB activity. USP16 deubiquitinates histone 2A (H2A), through which USP16 might regulate gene expression and cell cycle progression. Increased USP16 protein levels in people with DS might contribute to the development of certain phenotypes of DS.
Product Name:
6xHis-USP16
>Also Known As:
UBP-M; UBPM
>Catalog No.:
H6600
>Size:
25 µg
>Molecular Weight:
93.5 kDa
>Species:
Human
>Source:
Insect cell
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
>90% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_006447.2
>Image(s):
H6600 image
A. DUB activity assay: 15 nM USP16 + 500 nM Ub-Rhodamine 110. Rhodamine 110 fluorescence was monitored by a plate reader with excitation/emission wavelengths at 480/528 nm, respectively.
>Shipping Method:
Dry ice shipping
>References:
1. Joo H Y.,et al. (2007) Nature 449, 1068.
2. Adorno M., et al. (2013) Nature 501, 380. RVX-208 18469229
