6xHis-Sumo 2 (Q90P)
SUMO (small Ub-related modifier) is a Ub-like protein. Three types of SUMO are most commonly studied, SUMO 1, SUMO 2, and SUMO 3. SUMO 2 and SUMO 3 are almost identical isoforms and thus share many functions. Like Ub, SUMO can be conjugated to its target proteins as a polymeric chain. However, SUMO 1 forms chains inefficiently as compared to SUMO 2 and SUMO 3. SUMO is conjugated to target proteins by the E1 (SAE1/SAE2), E2 (Ube2I or Ubc9), E3 (RanBP2/Nup358, amongst others). Protein sumoylation is involved in many cellular processes including gene transcription.
The SUMO2(Q90P) mutant can still form polySUMO2 chains, but these chains are often resistant to desumolyation.
Product Name:
6xHis-Sumo 2 (Q90P)
>Also Known As:
HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; MGC117191
>Catalog No.:
E3250
>Size:
500 µg
>Molecular Weight:
10.9 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_006937
>Image(s):
N/A
>Shipping Method:
Dry ice shipping
>References:
1. Boddy MN, et al. (1996) Oncogene 13, 971 – 982.
2. Bayer P, et al. (1998) J Mol Biol 280, 275 – 286.
3. Melchior F, (2000) Annu Rev Cell Dev Biol 16, 591 – 626.
4. Praefcke GJK, et al. (2012) Trends Biochem Sci 37(1), 23 – 31.
5. Werner A, et al. (2012) Mol Cell 46(3), 287 – 298. BAY 61-3606 (dihydrochloride) 18478406
