6xHis-SAE1/SAE2
SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.
Product Name:
6xHis-SAE1/SAE2
>Also Known As:
SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091); SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)
>Catalog No.:
B1300
>Size:
25 µg
>Molecular Weight:
SAE1 (33 kDa), SAE2 (71.2 kDa)
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_005500 (SAE1); NM_002894 (SAE2)
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-SAE1/SAE2 |
>Shipping Method:
Dry ice shipping
>References:
1. Desterro JM, et al. (1999) J Biol Chem 274(15), 10618 – 10624.
2. Melchior F (2000) Annu Rev Cell Dev Biol 16, 591 – 626.
3. Schulman BA, et al. (2009) Nat Rev Mol Biol 10(5), 319 – 331. EW-7197 18474729
