6xHis-Nedd8
Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.
Product Name:
6xHis-Nedd8
>Also Known As:
NEDD-8, NEDD 8
>Catalog No.:
E4100
>Size:
500 µg
>Molecular Weight:
9 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_006156
>Image(s):
N/A
>Shipping Method:
Dry ice shipping
>References:
1. Kumar S, et al. (1993) Biochem Biophys Res Commun 195(1), 393 – 399.
2. Whitby FG, et al. (1998) J Biol Chem 273(52), 34983 – 34991.
3. Soucy TA, et al. (2010) Genes Cancer 1(7), 708 – 7016. AZD4547 1847865
