6xHis-CHIP
The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase. CHIP contains two domains, one is its TPR domain on the amino terminus and the other is its U-box domain on the carboxyl terminus. The TPR domain recognizes the chaperones, Hsp70 and Hsp90. While the U-box domain carries out its ubiquitin ligase activity. CHIP often ubiquitinates misfolded proteins bound on the chaperones.
Product Name:
6xHis-CHIP
>Also Known As:
STUB1; UBOX1; HSPABP2; NY-CO-7; SDCCAG7
>Catalog No.:
K1200
>Size:
50 µg
>Molecular Weight:
34.9 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_005861
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-CHIP |
>Shipping Method:
Dry ice shipping
>References:
1. Ballinger CA, et al. (1999) Mol Cell Biol 19(6), 4535 – 4545.
2. Murata S, et al. (2003) Int J Biochem Cell Biol 35(5), 572 – 578. GKT137831 18474876
